Coding
Part:BBa_K137014:Design
Designed by: Doug Tischer Group: iGEM08_Caltech (2008-06-20)
katE +LAA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1752
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Removal of an EcoRI site by site directed mutagenesis and addition of an ssrA tag with PCR primers
Source
E. coli genomic PCR and addition of ssrA tag with primers during PCR